The research objectives of the Zhong lab are to understand the
mechanisms of inflammatory diseases so that intervention and prevention
strategies can be developed. We have been using the upper genital tract
pathology induced by lower genital tract infection with chlamydial
organisms as a model to map chlamydial virulence factors and protective
antigens and to determine host pathways/effectors involved in either
pathogenesis or protective immunity.
Specific ongoing projects include: 1) Using proteomics and biochemical approaches for systematically
identifying and characterizing chlamydial pathogenic and protective
determinants. 2) Understanding the molecular mechanisms of chlamydial interactions
with hosts so that both chlamydial and host factors can be identified
as targets for attenuating chlamydial pathogenicity and/or candidates
for preventing chlamydial infection and diseases. 3) Investigating the roles of co-infection and vaginal microbiome and metabolome in chlamydial pathogenesis. 4) Transforming chlamydial organisms for delivering mucosal vaccines. 5) Screening for anti-fibrosis agents/approaches.
Huang Y, Zhang Q, Yang Z, Conrad T, Liu Y, Zhong G.Plasmid-Encoded Pgp5 Is a Significant Contributor to Chlamydia muridarum Induction of Hydrosalpinx. PLoS One. 2015 Apr 27;10(4):e0124840. doi: 10.1371/journal.pone.0124840.
Chen J, Yang Z, Sun X, Tang L, Ding Y, Xue M, Zhou Z, Baseman J, Zhong G.Intrauterine Infection with Plasmid-Free Chlamydia muridarum Reveals a Critical Role of the Plasmid in Chlamydial Ascension and Establishes a Model for Evaluating Plasmid-Independent Pathogenicity. Infect Immun. 2015 Jun;83(6):2583-92. doi: 10.1128/IAI.00353-15. Epub 2015 Apr
Sun X, Yang Z, Zhang H, Dai J, Chen J, Tang L, Rippentrop S, Xue M, Zhong G, Wu G.Chlamydia muridarum Induction of Glandular Duct Dilation in Mice. Infect Immun. 2015 Jun;83(6):2327-37. doi: 10.1128/IAI.00154-15. Epub 2015 Mar
Yang Z, Tang L, Sun X, Chai J, Zhong G.Characterization of CPAF Critical Residues and Secretion during Chlamydia trachomatis Infection. Infect Immun. 2015 Jun;83(6):2234-41. doi: 10.1128/IAI.00275-15. Epub 2015 Mar
Chen C, Zhou Z, Conrad T, Yang Z, Dai J, Li Z, Wu Y, Zhong G. In Vitro Passage Selects for Chlamydia muridarum with Enhanced Infectivity in Cultured Cells but Attenuated Pathogenicity in Mouse Upper Genital Tract. Infect Immun. 2015 May;83(5):1881-92. doi: 10.1128/IAI.03158-14. Epub 2015 Feb
Tang L, Yang Z, Zhang H, Zhou Z, Arulanandam B, Baseman J, Zhong G. Induction of protective immunity against Chlamydia muridarum intracervical infection in DBA/1j mice. Vaccine. 2014 Mar 10;32(12):1407-13.
Liu Y, Chen C, Gong S, Hou S, Qi M, Liu Q, Baseman J, Zhong G. Transformation of Chlamydia muridarum reveals a role for Pgp5 in suppression of plasmid-dependent gene expression. J Bacteriol. 2014 Mar;196(5):989-98.
Lei L, Dong X, Li Z, Zhong G. Identification of a novel nuclear localization signal sequence in Chlamydia trachomatis-secreted hypothetical protein CT311. PLoS One. 2013 May 23;8(5):e64529.
Li ZY, Huang QL, Su SM, Zhong GM, Wu YM. Development of ELISAs for the detection of urogenital chlamydia trachomatis infection targeting the pORF5 protein. Biomed Environ Sci. 2013 Mar;26(3):169-75.
Igietseme JU, Omosun Y, Partin J, Goldstein J, He Q, Joseph K, Ellerson D, Ansari U, Eko FO, Bandea C, Zhong G, Black CM. Prevention of Chlamydia-induced infertility by inhibition of local caspase activity. J Infect Dis. 2013 Apr;207(7):1095-104.