Research in the Stockand laboratory
focuses on ion channels and ion transport. We are particularly interested
in the proteins involved in controlling ion movement into neurons and
epithelial cells. The prior is important to excitability and how central
neurons signal to each other and how peripheral neurons allow us to sense our
environment. Ion channels in the kidney play a central role in
controlling blood pressure as they set circulatory volume and the water and
electrolyte content of plasma. We focus on understanding biologically
important mechanisms that affect physiology. The lab has a long
publication history on the mechanisms of regulation, structure-function
relations and physiologic function of Deg/ENaC ion channels, in which we have
employed electrophysiology, live-cell optical/fluorescence imaging, biochemical
and molecular biological approaches, as well as contemporary molecular genetics
and assessment of renal function, blood pressure and behavior in live animals.
Boiko N, Kucher V, Eaton BA, Stockand JD. Inhibition of neuronal degenerin/epithelial Na+ channels by the multiple sclerosis drug 4-aminopyridine. J Biol Chem. 2013 Mar 29;288(13):9418-27.
Stockand, JD. Chapter 8: Renal Ion Channels,
Electrophysiology of Transport and Channelopathies, in Seldin and Giebischâ€™s The Kidney: Physiology and Pathophysiology (Alpern,
R.J., Moe, O.W and Caplan, M. eds.) Elsevier Science, Amsterdam, The
Mironova E, Bugaj V, Roos KP, Kohan DE, Stockand JD. Aldosterone-independent regulation of the epithelial Na+ channel (ENaC) by vasopressin in adrenalectomized mice. Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):10095-100.
Boiko N, Kucher V, Stockand JD, Eaton BA. Pickpocket1 is an ionotropic molecular sensory transducer. J Biol Chem. 2012 Nov 16;287(47):39878-86.
Kucher V, Boiko N, Pochynyuk O, Stockand JD. Voltage-dependent gating underlies loss of ENaC function in Pseudohypoaldosteronism type 1. Biophys J. 2011 Apr 20;100(8):1930-9.